Plant Material Essay -- Plants, Seeds

Plant material The seeds of A. precatorius were collected from the medicative plant garden of Department of Pharmaceutical Sciences, Dr. H. S. Gour University, S agar, M.P., India. Seeds were sterilized and germinated by pursual the protocol described in our previous publication .15 Initiation of A. precatorius cellular telephone culturesDifferent explants from aseptically germinated seeds viz. leaves, epicotyle and petiole were tested for culture initiation by variation in plant growth regulators (PGR) and Agrobacterium mediated sack. Non- change indurate cultures were initiated by placing explants on loyalified MS medium supplemented separately with the hormones 1 mg/l naphthalene acetic acid (NAA) 1 mg/l Kinetin (Kn) 0.5 2.0 mg/l 2, 4- dichlorophenoxy acetic acid (2, 4-D) and there combinations (Data not shown). For transformation experiments, leaves were excised from 30 d old in vitro germinated plantlets of A. precatorius. A. tumefaciens strains (MTCC 431, MTCC 609, MTCC 2250 and MTCC 2251) were used to establish transformed callus cultures. These strains were procured from microbial Type Culture Collection (MTCC), Institute of Microbial Technology (IMTECH), Chandigarh, India. A minimum of 30 explants were used for each experiment. solely explants cultured on sterilized petriplates comprising MS medium solidified with 1.0 % agar and supplemented with 30 g/l sucrose. The pH was adjusted to 5.7 0.2. The medium was autoclaved downstairs 15 psig pressure at 121C for 20 min. The explants were co-cultivated with Agrobacterium strains for transmission to induce transformed callus. For this purpose, Agrobacterial colonies were cultured for 48 h on solid nutrient agar medium at 28 2C. ten loopful bacteria were then... ... in a maximum synergistic promotion of glycyrrhizin collecting i.e. 4.9-fold higher compared to transformed control culture. The present study indicates the potential of these biotechnology-based methodologies for big pro duction of glycyrrhizin. Furthermore, in order to develop a process for commercial production of glycyrrhizin by plant cell cultures some additional soften enhancement strategies may be worked out like, optimization of medium composition, environmental condition and addition of precursors.Acknowledgments The authors are thankful to Dr. Ashish Baldi, Department of Biochemical engineer and Biotechnology, Indian Institute of Technology, Hauz Khas, New Delhi, India for his valuable and timely assistance. The author VSK wishes to be intimate All India Council for Technical Education, New Delhi for providing junior research scholarship.